TDZ AND 4-CPPU in Gamborg B5 salts with MS vitamins doubles embryogenic 191 response from male flowers of EA-AAA banana.

Abstract

Conventionally, auxins have been used in MS medium in combination or without purine-based cytokinins for induction of embryogenesis in EA-AAA banana (Musa spp.). Besides, low embryogenic response, it has been rare for more than two cultivars to respond similarly to a single treatment. This study investigated the efficacy of urea-type cytokinins, N-phenyl-N’-1,2,3-thidiazol-5-ylurea (TDZ) and N-(2-chloro-4-pyridyl)-N'-phenylurea (4-CPPU); and salt formulations, Chu (N6), Eriksson, Gamborg B5, MS, Nitsch, NLN, SH and White for embryogenic callus induction in different EA-AAA banana cultivars. Immature male flowers of cultivars Mpologoma, Mbwazirume, Nakabululu, Nakinyika and Nfuuka were cultured on callus induction medium, supplemented with different TDZ and 4-CPPU combinations. Most of the cultivars had embryogenic response to the medium with 10μM TDZ+10μM CPPU. Cultivar Nakabululu recorded 22.2% embryogenic response, followed by Mwazirume (5.7%), Nakinyika (5.3%) and Mpologoma (4.6%). Cultivar Nfuuka had 9.1% embryogenic response on 15μM TDZ+15μM CPPU. When cultivars Mpologoma and Nakinyika were cultured on the same medium containing 10μM TDZ+10μM CPPU, but the MS salts substituted with the other salt formulations, their cultures recorded 11.4 and 8.3% embryogenic response, respectively to Gamborg B5 salts; which was almost twice their response to MS medium. The results suggested that TDZ and 4-CPPU, particularly in Gamborg B5 salt formulation, could increase percentage of embryogenic callus induced from male flowers of EA-AAA banana cultivars, and would improve plant regeneration and consequently help in the process of genetic improvement of EA-AAA banana.

Key Words: Cytokinins, embryogenic response, Musa spp., Thidiazuron