Plant regeneration and stimulation of <i>in vitro</i> flowering in <i>Eruca sativa</i> Mill.

Abstract

Explants such as apical buds, axillary buds, cotyledons, cotyledonary nodes, leaves, hypocotyls and immature embryonal axes from in vitro-grown plantlets were inoculated on the Murashige and Skoog (MS) medium supplemented with 4.44 μM 6-benzylaminopurine in combination with 2.85 μM indole-3-acetic acid. Best multiple shoots formation was obtained with cotyledonary nodes. Each inoculated explant produced 18.10 ± 0.66 shoots within 2 to 3 weeks. These shoots were separated carefully and were transferred to the fresh half strength MS solid medium with indole-3-butyric acid (4.90 μM) for the development of the roots. These in vitro-developed plantlets produced flowers on the same medium with supplementation of 6-furfuril kinetin (0.23 μM). These plantlets were successfully transferred to the soil where they grew well for 8 to 10 weeks with 80% survivability.

Keywords: Eruca sativa, cotyledonary nodes, in vitro regeneration, in vitro flowering, shoot multiplication