Article Sidebar
Article Details
Copyright: Creative Commons Attribution CC.
This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials. View License Deed | View Legal Code Authors can also self-archive their manuscripts immediately and enable public access from their institution's repository. This is the version that has been accepted for publication and which typically includes author-incorporated changes suggested during submission, peer review and in editor-author communications.
Main Article Content
In vitro wound healing activity of <i>Sapium indicum</i> willd leaf extracts.
Abstract
Background: Sapium indicum Willd (Euphorbiaceae) is traditionally used to accelerate wound healing process in Malay community.
Objective: To evaluate wound healing potential of aqueous (AESI) and ethanolic extract of S. indicum leaves (EESI) using cell migration model.
Methods: AESI and EESI were prepared using maceration techniques. The extracts were subjected to phytochemical screening, cytotoxicity (MTT assay) and scratch assay.
Results: AESI contained saponins and tannins while EESI contained triterpenes, tannins and proteins. Based on MTT assay, AESI and EESI
exhibited IC50 = 16.3 and 70.9 μg/mL, respectively. In the scratch assay, EESI caused significant (P< 0.05) concentration-dependent migration
on 3T3 L1 cells whereas AESI exerted concentration-independent effect.
Conclusion: The leaves of S. indicum exhibited wound healing potential and contained phytochemicals that may contribute to the activity. These
findings would support potential use of S. indicum as wound healing plant.
Keywords: Sapium indicum, cell migration, in vitro wound healing
