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Investigation into the influence of certain physico-chemical parameters on the distribution of macrobenthic invertebrates in Woji creek was done between April, 2001 and March, 2002. Temperature of the sub-surface water ranged from 25.5°C to 30.9°C. The degree of hydrogen ion concentration, pH varied between 5.5-7.8. Salinity showed spatial variation ranging from 012.7%₀ while Dissolved Oxygen Concentration (DO) had values between 1.6mg/l and 10.1mg/l. A total of thirty taxa of benthic macroinvertebrates were encountered. These belong to 20 families within 5 classes. Regression analyses revealed that pH significantly (P