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The impact of short-term exposure to waterborne chlorpyrifos-ethyl and lambda-cyhalothrin on Clarias gariepinus was evaluated through changes of selected biochemical parameters. Fish was exposed to 0.64 mg/l, 0.80 mg/l, 0.96 mg/l, 1.12 mg/l, 1.28 mg/l of chlorpyrifos-ethyl, and 0.008mg/l, 0.009mg/l, 0.010mg/l, 0.011mg/l, 0.012mg/l of lambda-cyhalothrin and control for 96hrs. The 96-h LC50 value for C. gariepinus was estimated as 0.92mg/l and 0.008mg/l for chlorpyrifos-ethyl and lambda-cyhalothrin respectively. The parameters measured were serum glucose, protein, cholesterol, triglyceride, glutamic pyruvic acid transaminase (GPT), glutamic oxaloacetic acid transaminase (GOT) and alkaline phosphatase (ALP).There was significant (P