In vitro antimicrobial evaluation of methanol extract of Triumfetta rhomboidea leaves against some clinical bacterial isolates

Abstract

The antimicrobial activity of methanol extract of Triumfetta rhomboidea leaves was evaluated against Staphylococcus aureus, Salmonella typhi and Klebsiella pneumoniae. Agar diffusion and agar dilution techniques were employed for the antimicrobial sensitivity and interaction studies. Phytochemical analysis of the crude extract was also carried out using standard procedures. The results obtained showed that the crude extract exhibits a substantial antimicrobial activity against Klebsiella pneumoniae (which recorded the greatest sensitivity), Salmonella typhi but not against Staphylococcus aureus. The result of the interactive studies between the crude extract and standard antibiotics showed relationship ranging from antagonism to synergy. Phytochemical analysis revealed the presence of terpenes, flavonoids and other phenolics and resins in the crude extract. Thus, this study shows that Triumfetta rhomboidea possesses promising antimicrobial activity especially against Klebsiella pneumoniae.

Keywords: Triumfetta rhomboidea, Antimicrobial activity, Microorganisms, Susceptibility, Resistance, Interaction