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Prospective study on antimicrobial protein of <i>Spirastrella inconstans</i> against Methicillin Resistant <i>Staphylococcus aureus</i> (MRSA)
Abstract
The bioactive compound have to be extracted from the marine sponge Spirastrella inconstans for treatment against Methicillin Resistant Staphylococcus aureus (MRSA), which were isolated from the chronic
wound of fisherman community of east and west coastal villages of India. About 100 MRSA strains were isolated from the pus samples and confirmed using standard biochemical tests. Since those strains developed resistance to almost all type of antibiotics, alternative bioactive compounds are needed for the treatment. Spirastrella inconstans was selected in which the crude bioactive compound were extracted by solvent extraction method. The antimicrobial activity was performed using agar well cutting and paper disc diffusion methods, in which chloroform extract was the most active compared to that of other solvents, with a maximum of 30 mm diameter of zone of inhibition. That crude extract protein was estimated by using Lowry’s method. The protein was precipitated using ammonium sulphate precipitation method and purified using dialysis. The purified protein was found to be of 3,000 Da molecular weight by SDS-PAGE, it can be used for the treatment of wound infection though the structural and molecular elucidations need to be done in the future.
wound of fisherman community of east and west coastal villages of India. About 100 MRSA strains were isolated from the pus samples and confirmed using standard biochemical tests. Since those strains developed resistance to almost all type of antibiotics, alternative bioactive compounds are needed for the treatment. Spirastrella inconstans was selected in which the crude bioactive compound were extracted by solvent extraction method. The antimicrobial activity was performed using agar well cutting and paper disc diffusion methods, in which chloroform extract was the most active compared to that of other solvents, with a maximum of 30 mm diameter of zone of inhibition. That crude extract protein was estimated by using Lowry’s method. The protein was precipitated using ammonium sulphate precipitation method and purified using dialysis. The purified protein was found to be of 3,000 Da molecular weight by SDS-PAGE, it can be used for the treatment of wound infection though the structural and molecular elucidations need to be done in the future.
