The nutritional suitability and cost effectiveness of cottonseed meal (CSM) as protein source in the diet of Nile tilapia (Oreochromis niloticus L.) with mean initial weight 4.24±0.20 g, was evaluated over a 56-day growth period. Four isonitrogenous (320 g kg-1), isolipidic (100 g kg-1) and isoenergetic (18 KJ g-1) test diets were formulated in which CSM protein replaced fish meal (FM) protein at levels of 0% (control), 25%, 50% and 75%. The control diet had FM as the sole protein source. The growth experiment was conducted in plastic tanks in a recirculation system each dietary treatment was in triplicate. After 56 days of feeding fish at 6% - 4% body weight per day, CSM protein replacements of 25% and 50% did not significantly (P