A study was conducted at National Veterinary Research Institute (NVRI) Animal Health Research laboratory to determine serum total fatty acids in  dermatophilosis resistant and susceptible (healthy and infected) cattle breeds in Vom. A total of 30 Cattle, comprising 15 resistant (7 N’dama and 8 Muturu) and 15 susceptible (8 healthy (4 Sokoto Gudali and 4 White Fulani) and 7 infected (3 Sokoto Gudali and 4 White Fulani) animals. Blood samples were collected from the Jugular vein using 18 gauge hypodermic needle and decanted into sterile sample bottles. The bottles were properly labeled for proper identification and immediately submitted to the laboratory for serum fatty acids analysis. The results obtained from the laboratory, were subjected to statistical analysis using T- Test for unpaired observations. The results showed that serum total fatty acid were significantly higher (p< 0.05) in resistant cattle than the susceptible ones. Also, there was significantly higher (p