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Hypoglycaemic And Alpha-Amylase Inhibitory Activities Of Fermented Seeds Of Parkia Biglobosa (Jacq) Benth
AA Fred-Jaiyesimi, OO Olubomehin, RM Wilkins, KO Abo
Abstract
Following our continuing search for plant anti-diabetic agents, we report results of a bioactivity-guided fractionation of fermented seeds of Parkia
biglobosa used in ethnomedicine in South West Nigeria to manage diabetes mellitus. An active aqueous MeOH macerate was separated by solvent partition and the bioactive CHCl3 extract fractionated on column chromatography packed with silica gel G60A, eluting with gradient mixtures of Pet ether, EtOAC and MeOH.Biologically monitored separation of extracts was achieved using alloxan-diabetic rats, glucose loading (1g/kg) and alpha-amylase inhibition assays as basis for identification of active constituent. Peak reduction (48.5%, P
biglobosa used in ethnomedicine in South West Nigeria to manage diabetes mellitus. An active aqueous MeOH macerate was separated by solvent partition and the bioactive CHCl3 extract fractionated on column chromatography packed with silica gel G60A, eluting with gradient mixtures of Pet ether, EtOAC and MeOH.Biologically monitored separation of extracts was achieved using alloxan-diabetic rats, glucose loading (1g/kg) and alpha-amylase inhibition assays as basis for identification of active constituent. Peak reduction (48.5%, P
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Nigerian Journal of Natural Products and Medicine. ISSN: