Summary: The study investigated the effects of caffeine and ethanolic extract of kolanut (EEK) on glucose uptake in the canine hindlimb at rest and during contraction. Thirty male anaesthetized Mongrel dogs (11 - 13kg) were divided into six groups (5dogs/group). Caffeine (6mg/kg), EEK (5mg/kg), or normal saline (control) was administered intravenously to each group at rest. Arterial and venous blood samples were collected at 0, 5, 10, 15, 20, 25, 30, 45, 60, 75 and 90 minutes after drug administration. Blood glucose was measured by glucose oxidase method. Arterio-venous (A-V) glucose difference was calculated and venous
blood flow (VBF) was measured during the sampling period. Hindlimb Glucose Uptake (HGU) was calculated as the product of (A-V) glucose and blood flow. After sampling at rest, the experiments were repeated with the right femoral nerve stimulated using electrical stimulator at 5Hz. At rest, (A-V) glucose increased significantly (P