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In vitro inhibition of drug-resistant and drug-sensitive strains of Mycobacterium tuberculosis by Helichrysum caespititium
JJM Meyer, N Lall, ADM Mathekga
Abstract
Antimycobacterial activity of acetone and water extracts of Helichrysum
caespititium against a drug-sensitive strain of Mycobacterium tuberculosis was evaluated
by the agar plate method. The acetone extract exhibited inhibitory activity
at a concentration of 0.5mg ml-1 against this strain whereas the
organism was found to be partially susceptible to the water extract at 5.0mg
ml-1. The inhibitory
activity of the acetone extract was confirmed using the rapid radiometric method
and the minimum inhibitory concentration (MIC) was found to be 0.1mg ml-1.
Caespitate, a novel phloroglucinol, which was previously isolated and identified,
from H. caespititium was also evaluated for its activity against drug-sensitive
and drug-resistant strains of M. tuberculosis. The MIC of caespitate was found
to be 0.1mg ml-1 for all the M. tuberculosis strains. The ability of caespitate
to inhibit the growth of all the strains of M. tuberculosis, shows the broad
spectrum antimycobacterial activity of the compound.
South African Journal of Botany 2002, 68: 90–93
caespititium against a drug-sensitive strain of Mycobacterium tuberculosis was evaluated
by the agar plate method. The acetone extract exhibited inhibitory activity
at a concentration of 0.5mg ml-1 against this strain whereas the
organism was found to be partially susceptible to the water extract at 5.0mg
ml-1. The inhibitory
activity of the acetone extract was confirmed using the rapid radiometric method
and the minimum inhibitory concentration (MIC) was found to be 0.1mg ml-1.
Caespitate, a novel phloroglucinol, which was previously isolated and identified,
from H. caespititium was also evaluated for its activity against drug-sensitive
and drug-resistant strains of M. tuberculosis. The MIC of caespitate was found
to be 0.1mg ml-1 for all the M. tuberculosis strains. The ability of caespitate
to inhibit the growth of all the strains of M. tuberculosis, shows the broad
spectrum antimycobacterial activity of the compound.
South African Journal of Botany 2002, 68: 90–93
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South African Journal of Botany. ISSN: 0254-6299