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Research Note
Somatic embryos can be induced from the vegetative shoot apex of mature Pinus patula trees
Ravindra B Malabadi, J van Staden
Abstract
Embryogenic cultures were initiated and established and somatic embryos produced in Pinus patula using thin sections from the dome of the vegetative shoot apex.
Factors affecting initiation, basal medium and plant growth regulators were
investigated. We report for the first time the initiation of embryogenic tissue
and embryos from shoot apical domes (zones) of 15-year-old Pinus patula
trees. The growth of embryogenic lines were comparable to those obtained from zygotic embryos. Plantlets were regenerated.
South African Journal of Botany 2003, 69:
450–451
Factors affecting initiation, basal medium and plant growth regulators were
investigated. We report for the first time the initiation of embryogenic tissue
and embryos from shoot apical domes (zones) of 15-year-old Pinus patula
trees. The growth of embryogenic lines were comparable to those obtained from zygotic embryos. Plantlets were regenerated.
South African Journal of Botany 2003, 69:
450–451
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South African Journal of Botany. ISSN: 0254-6299