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The acute toxicity of fenitrothion (FNT) alone and combined with piperonyl butoxide (PBO) or triphenyl phosphate (TPP), and their effect on liver esterase (LE) activity of Gambusia holbrooki, Pseudorasobora parva and Oncorhynchus mykiss was studied in aquaria for 96h. The results indicated that fenitrothion is moderately toxic to the three species of fish tested, the toxicity being highest to O. mykiss followed by P. parva and G. holbrooki. Both FNT concentrations and the exposure time on the activity of LE and FNT (two way ANOVA P