Tukuyu onchocerciasis focus was earmarked for vector control using insecticide against larval stages.
Susceptibility tests of mature larvae of Simulium damnosum s.l. vectors to temephos insecticide were carried out before
and after two years of insecticide treatment of rivers within Tukuyu onchocerciasis focus, south-western Tanzania. The
tests were done in 1999/2000 and 2004 using WHO standard methods. Mature larvae were exposed to 9 concentrations of
temephos active ingredient, from the weakest 0.00975mg/litre to the strongest of 2.5mg/l. Each test concentration and
control was run in duplicates of 25 larvae each, set for three hours in a cool temperature. After incubation, test solution was
discarded and larval condition checked. Numbers of larvae in each category were recorded and used to determine mortality
rate for each concentration as well as for the LC50 and LC95. A total of 1,666 larvae were tested, 942 during the pre- and 724
post-treatment. Results showed that both pre and post-treatment samples were susceptible, attaining 100% mortality at the
diagnostic dose of 1.25mg/l, and LC50 between 0.129-0.34mg/l pre - and 0.144-0.211 mg/l (95% CI, P