Occurrence of diarrhoea and other water-borne diseases in cities of developing countries has been on the increase over the decades largely due to unsafe water, inadequate sanitation and poor hygiene among human population. This study examined the relationship between the spatial pattern of water-borne diseases vis-a- vis water quality in parts of Ibadan City in Nigeria. Data on 1,334 cases of various water-borne diseases was collected from eight public hospitals with catchments covering the study area. Three areas with relatively high incidence of water-borne diseases and another three areas with relatively low incidence were selected for water sampling and household survey. Water samples from rains, wells, and borehole were collected for physical and bacteriological analyses. A well structured questionnaire was administered to 350 residents of the selected areas to elicit information on water sourcing, handling and storage. Analysis of hospital records showed significant difference in the occurrence of water-borne diseases among residential areas (P