The prevalence of typanosome infection in 65 cattle reared under expensive system of management was determined using the dark ground buffy coat (DG) technique and the enzyme-linkedimmunisorbent assay (ELISA). The DG technique showed that there were 18 positive cases (27.69%) of total number of animals, made up of 6 of Trypanosoma congolense, 7 of T. Vivax and 3 of T. Brucei. Other cases were those of mixed infections mainly one case of which all of 3 Trypansomes species were present, and another in which T. vivax, 12 of T. brucei and 2 cases of T. brucei were 30, (46.15%) positive cases made up of 6 of T. congolense, 7 of T. vivax, 12 of T. brucei and 2 cases of T. brucie mixed with t. Conglolese. The ELISA technique did not detect any incidence of mixed infections in which 3 species of trypanosomes were present.

Key words: ELISA, Buffy coat, Trypanosomosis, cattle