Article Sidebar
Published:
Sep 9, 2014Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
<i>Olea europaea</i> Linn (Oleaceae) Fruit Pulp Extract Suppresses Sterol Regulatory Element-Binding Proteins-1c via AMP-Activated Protein Kinase Activation in Human Hepatic Cells
Abstract
Purpose: To investigate the molecular mechanisms underlying the role of Olea europaea Linn (Oleaceae) fruit pulp extract (OPF) in the prevention of high glucose-induced lipid accumulation in human HepG2 hepatocytes.
Methods: HepG2 cells were pretreated with various concentration of OPF (0, 10, 20, 40 and 80 µg/ml) and then treated with serum-free medium with normal glucose (5 mM) for 1 h, followed by exposure to high glucose (25 mM D-glucose) for 24 h. Cell viability and cytotoxicity parameters were measured using LDH and MTT assay while triglyceride and total cholesterol levels were evaluated using respective enzymatic reagent kits. Protein expressional levels were analyzed by Western blotting.
Results: OPF did not influence the cell viability (up to 200 µg/ml) and showed any signs of cytotoxicity (up to 80 µg/ml). OPF significantly attenuated lipid accumulation in human HepG2 hepatocytes when
exposed to high glucose (25 mM D-glucose) in a dose-dependently (p < 0.05, 0.01 and 0.001 at 20, 40 and 80 µg/ml concentrations, respectively). Nile red staining showed that 10, 20, 40 and 80 µg/ml concentrations of OPF reduced lipid accumulation by 25, 33.7 (p< 0.05), 43.7 (p attenuated by OPF (p < 0.001 at 80 µg/ml). Further, OPF attenuated the expression of fatty acid synthase and sterol regulatory element-binding protein-1. Adenosine monophosphate-activated protein kinase was also activated by OPF treatment when exposed to high glucose (25 mM D-glucose) in
human HepG2 hepatocytes.
Conclusion: This study indicates that OPF has hypolipidemic effects by inhibiting lipid biosynthesis mediated via AMPK signaling. Thus, this extract can be potentially developed into an anti-obesity agent.
Keywords: Olea europaea, Fruit pulp extract, Lipogenesis, AMPK SREBP-1, Anti-obesity; HepG2 cells
