Article Sidebar
Published:
Nov 14, 2016Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Induction of protective immune responses in mice by double DNA vaccine encoding of <i>Brucella melitensis</i> Omp31 and <i>Escherichia coli</i> Eae genes
Abstract
Purpose: To investigate the efficacy of a double DNA vaccine encoding of Brucella melitensis omp31 gene and of Escherichia coli eae gene in inducing protective immune response in a mouse model.
Methods: After performing PCR assays and cloning both the eae and omp31 genes, the generated DNA vaccines were intramuscularly injected into BALB/c mice. Purified proteins and transformed cell lysates were identified by western blot and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and analyzed by enzyme-linked immunosorbent assay (ELISA).
Results: pcDNA3-omp31-eae provokes a significant, high level of protection in BALB/c mice against challenges with E. coli and B. melitensis. Titers ranging from 90:160 to 2,570:5,110 were detected 60 days after vaccination. Notably, rOmp31 and rEae proteins significantly induced the production of IFN-γ in cells from pcDNA3-omp31-eae-immunized mice (p < 0.05 in both groups) and only low levels of IL-10 were found in all groups. The lymphocytes from mice immunized with pcDNA3-omp31-eae had an apparent T cell proliferative response to rOmp31 and rEae proteins (p < 0.01) with a stimulation index of 14.90.
Conclusion: The novel pcDNA3-omp31-eae construct can be used as a DNA vaccine to prevent E. coli and B. melitensis infections in future studies.
Keywords: Multiple DNA vaccine, Omp31 gene, Brucella melitensis, Eae gene, Escherichia coli, Protective immune response
