In vivo hypoglycemic effect of methanolic fruit extract of Momordica charantia L

  • W Nkambo
  • NG Anyama
  • B Onegi

Abstract

Background: Momordica charantia L. is a medicinal plant commonly used in the management of diabetes mellitus.
Objectives: We investigated the blood glucose lowering effect of the methanolic fruit extract of the Ugandan variety of M. charantia L. in alloxan-induced diabetic albino rats.
Methods: 500g of M. charantia powder were macerated in methanol and the extract administered to two groups of alloxaninduced diabetic rats. The first group received 125mg/kg, the second 375mg/kg and a third group 7mg/ kg of metformin. A fourth group received 1ml normal saline. Fasting blood glucose (FBG) levels were measured at 0.5,1,2,3,5,8 and 12 hours and compared using one-way ANOVA. Results: There was an initial rise in FBG for 1 hour after administration of extracts followed by steep reductions. Significant reduction in FBG occurred at 2 hours for 125mg/kg of extract (-3.2%, 313±25.9 to 303±25.0mg/dL, p = 0.049), 375mg/kg of extract (-3.9%, 356±19.7 to 342±20.3mg/dL, p = 0.001), and metformin (-2.6%, 344±21.7 to 335±21.1mg/dL, p = 0.003) when compared to normal saline. The maximum percentage reduction in FBG by both extracts occurred between 3 and 12 hours post dose.
Conclusions: The methanolic fruit extract of M. charantia exhibits dose dependent hypoglycaemic activity in vivo.

Keywords: Momordica charantia, methanolic extract, Diabetes Mellitus, hypoglycaemic effect, in vivo

African Health Sciences 2013; 13(4): 933 - 939

Author Biographies

W Nkambo
Department of Pharmacy, School of Health Sciences, College of Health Sciences, Makerere University, P.O. Box 7072, Kampala, Uganda
NG Anyama
Department of Pharmacy, School of Health Sciences, College of Health Sciences, Makerere University, P.O. Box 7072, Kampala, Uganda
B Onegi
Department of Pharmacy, School of Health Sciences, College of Health Sciences, Makerere University, P.O. Box 7072, Kampala, Uganda
Published
2014-01-29
Section
Articles

Journal Identifiers


eISSN: 1680-6905