Genetic diversity and gene flow in Clarias gariepinus from Lakes Victoria and Kanyaboli, Kenya

  • JE Barasa
  • R Abila
  • JP Grobler
  • OG Dangasuk
  • MN Njahira
  • B Kaunda-Arara

Abstract

The African catfish Clarias gariepinus is an important species in the rapidly expanding aquaculture industry in East Africa. Most Kenyan catfish farmers use stocks with unknown genetic characteristics, with uncertified seeds and inter-basin exchange of brood stocks threatening the genetic distinctness of wild populations. Using 346 base pairs of D-loop sequence variation, genetic diversity and gene flow between C. gariepinus populations from Lake Victoria and its satellite, Lake Kanyaboli, were explored. A total of 17 haplotypes were identified in 52 individuals sampled, with the two populations sharing four haplotypes, and one haplotype being the most frequent (50%) in both populations. Catfish from Lake Victoria showed marginally higher genetic variation compared to those from Lake Kanyaboli, reflected in the higher number of haplotypes, singletons, polymorphic sites and haplotype and nucleotide diversities. Yet neither population showed signs of significant loss of diversity compared to other wild populations of the species. Clarias gariepinus from Lakes Victoria and Kanyaboli clustered into one clade, showing low population structuring and with a between-population FST value of 0.026, which was not indicative of significant (p ≥ 0.05) differentiation between the two lakes. Nevertheless, each population contained 60–64% of unique haplotypes. Inter-basin transfer of Clarias populations and human impact on Lake Kanyaboli should be controlled to conserve the unique Clarias genetic resources in the lake basin of Kenya.

Keywords: African catfish, conservation, genetic variation, haplotype, population differentiation

African Journal of Aquatic Science 2014, 39(3): 287–293

Author Biographies

JE Barasa
Department of Fisheries and Aquatic Sciences, University of Eldoret, Eldoret, Kenya; Current address: Ministry of Fisheries Development, Eldoret, Kenya
R Abila
Department of Fisheries Management and Aquaculture Technology, South Eastern Kenya University, Kitui, Kenya
JP Grobler
Department of Genetics, University of the Free State, Bloemfontein, South Africa
OG Dangasuk
Department of Biological Sciences, Molecular Genetics Section, University of Eldoret, Eldoret, Kenya
MN Njahira
Biosciences eastern and central Africa - International Livestock Research Institute Hub (BecA-ILRI Hub), Nairobi, Kenya
B Kaunda-Arara
Department of Fisheries and Aquatic Sciences, University of Eldoret, Eldoret, Kenya
Published
2014-11-07
Section
Articles

Journal Identifiers


eISSN: 1727-9364
print ISSN: 1608-5914