Microcystin-LR equivalent concentrations in fish tissue during a postbloom Microcystis exposure in Loskop Dam, South Africa

  • T Nchabeleng
  • P Cheng
  • PJ Oberholster
  • A-M Botha
  • WJ Smit
  • WJ Luus-Powell

Abstract

The effects of a decomposing cyanobacteria bloom on water quality and the accumulation of microcystin-LR equivalent toxin in fish at Loskop Dam were studied in May 2012. Enzyme-linked immunosorbent assay [ELISA] was used to confirm the presence of microcystin-LR equivalent in the water and to determine the microcystin (MCYST) concentration in the liver and muscle of fish. The lowest concentration of extracellular MCYST-LR equivalent was recorded in the lacustrine zone, where no cyanobacterial cells were observed, while the highest concentration (3.25 μg l−1), 3.25x higher than World Health Organization standard, was observed in the riverine zone. Extremely high MCYST-LR equivalent concentrations of 1.72 μg MCYST-LReq kg−1 in the liver and 0.19 μg kg−1 in muscles of Labeo rosae, and 2.14 μg MCYST-LReq kg−1 in the liver and 0.17 μg kg−1 in muscles of Oreochromis mossambicus, indicate that the consumption of sufficient fish biomass might cause severe adverse effects in humans. Microscopic analyses of the stomach content of both fish species revealed low numbers of cyanobacterial Microcystis aeruginosa cells in comparison to other phytoplankton. The extracellular MCYST-LR equivalent of the decomposing bloom may have played a major role in the high levels observed in the livers of the two fish species. These findings are important for all downstream water users.

Keywords: cyanobacteria, indigenous fish species, Labeo rosae, liver, muscle tissue, Oreochromis mossambicus

African Journal of Aquatic Science 2014, 39(4): 459–466

Author Biographies

T Nchabeleng
Department of Biodiversity, School of Molecular and Life Sciences, University of Limpopo, Sovenga, South Africa
P Cheng
CSIR Natural Resources and the Environment, Stellenbosch, South Africa
PJ Oberholster
CSIR Natural Resources and the Environment, Stellenbosch, South Africa; Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa
A-M Botha
Department of Genetics, University of Stellenbosch, Stellenbosch, South Africa
WJ Smit
Department of Biodiversity, School of Molecular and Life Sciences, University of Limpopo, Sovenga, South Africa
WJ Luus-Powell
Department of Biodiversity, School of Molecular and Life Sciences, University of Limpopo, Sovenga, South Africa
Published
2015-04-01
Section
Articles

Journal Identifiers


eISSN: 1727-9364
print ISSN: 1608-5914