The effect of nifedipine induced calcium antagonism on in vitro toxicity of lindane
The study of the effect of calcium antagonist (nifedipine) on isolated rabbit ileum poisoned with lindane was conducted. Rabbits of 1.2kg average body weight which was obtained from the Department of Pharmacology, Ahmadu Bello University, Zaria animal house were used for the current study. Each rat was sacrificed by a blow on the head, dislocation of the neck and exsanguinations. The abdomen was opened up and 4cm in length segment of the ileum obtained and mounted in an isolated organ bath containing Tyrodes solution. The preparation was maintained at 37oC aerated with air. (Kitchen, 1984; Dede, et al, 1991). 2 m g/ml lindane attenuated acetylcholine (1m g/ml) response, significantly different from acetylcholine response alone. P<0.05, P<0.01 in normal Tyrode's solution and Ca2+ free Tyrode's solutions respectively. 5m g/ml nifedipine attenuated acetylcholine response significantly different from acetylcholine response alone (P<0.05 in normal Tyrode's solution and Ca2+ free Tyrode's solution respectively. 5m g/ml nifedipine augmented reduction of acetylcholine response with 2m g/ml lindane significantly different from acetylcholine response alone P<0.01, and P<0.001 in normal Tyrode's solution and Ca2+ free Tyrode's solution respectively. It was concluded that nifedipine (calcium channel blocker) augmented inhibitory action of lindane on acetylcholine response.
AJAZEB Vol. 7 2005: pp. 28-31