Factors influencing callus induction and plant regeneration of Dahurian wildrye grass (<i>Elymus dahuricus</i> L.)

Abstract

Establishment of highly efficient and reproducible regeneration system would greatly influence the efforts of improvement of Elymus sp. through gene transfer technology. A suitable callus induction and efficient regeneration protocol for Dahurian wildrye grass (Elymus dahuricus L.) was developed. It consisted of 3.0 mg/L 2,4 dichlorophenoxyacetic acid (2,4-D) or Dicamba + 1.0 g/L casein hydrolysate (CH) + 300 mg/L L-proline + 30 g/L sucrose in MS, and showed the highest percentage of callus induction. Highest (51%) regeneration was obtained from N6 medium containing 1.0 mg/L 2,4-D + 3.0 mg/L N₆-benzylaminopurine (BA) + 1.0 g/L CH + 300 mg/ L L-proline + 30 g/L sucrose. Thus, optimization of regeneration frequency using mature seeds as explants material may offer a simple and efficient protocol for Dahurian wildrye grass that may improve molecular breeding of this species.

Key words: Dahurian wildrye grass, Elymus dahuricus L., callus, plant regeneration.