Identification of different selected genotypes of salinity resistance of each of full-sib Nile tilapia, Blue tilapia and their diallel interspecific hybridization using random amplified polymorphic DNA fingerprinting

Abstract

Random amplified polymorphic DNA (RAPD) analysis was applied to identify the three selected genotypes which were defined as; low, medium and high salinity resistance of each of full-sib Nile tilapia (Oreochromis niloticus), Blue tilapia (Oreochromis aureus) and their diallel interspecific hybridization. Six random primers were used to assay polymorphisms among the different selected genotypes within each species or hybrid. The results reveal that high genetic polymorphic percentages were detected among low, medium and high salinity resistance of different selected genotypes within each species or hybrid using different random primers. The results suggest also that RAPD technique can be successfully used as a rapid and easy way for identification of the different selected genotypes of salinity resistance fish, which considers a great potential for the development and implementation of genetic improvement programs.

Keywords: RAPD fingerprinting, salinity resistance, Nile tilapia, Blue tilapia, interspecific hybridization