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Body extract of tail amputated zebrafish promotes culturing of primary fin cells from glass catfish


Jee Eun Han
Ji Hyung Kim
Casiano Choresca
Sang Phil Shin
Jin Woo Jun
Se Chang Park

Abstract

The most spectacular regenerative events in vertebrates are epimorphic regeneration. In this study, interestingly, a whole-body extract 24 h after tail amputation enhanced primary cell growth and viability compared to that of a non-tail amputated body. Additionally, these effects of extract treatment in vitro were dose-dependent occurring at concentrations of 0.02, 0.05 and 0.1 mg/ml. This is the first in vitro study on the interaction between primary fin cells from glass catfish and tail amputated body extracts of zebrafish. These results provide an essential knowledge base for rational approaches to tissue and organ regeneration.

Keywords: Cell growth, cell viability, extract, glass catfish, regeneration, zebrafish

African Journal of Biotechnology Vol. 12(12), pp. 1449-1451

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eISSN: 1684-5315