Plant regeneration protocol of <i>Andrographis paniculata</i> (Burm. f.) - an important medicinal plant

Abstract

Rapid direct plant regeneration of Andrographis paniculata was achieved from leaf and stem explants on Murashige and Skoog (MS) basal medium supplemented with 1.5 to 3.0 mg/l 6-benzyladenine (BA), 50 mg/l adenine sulfate (Ads) and 3% (m/v) sucrose. Inclusion of 1.0 mg/l 1-naphthalene acetic acid (NAA) in the culture medium along with BA + Ads promoted a higher rate of shoot bud regeneration. Maximum mean number of shoot bud per explant (28.6) was achieved on the MS medium supplemented with 3.0 mg/l BA, 50 mg/l Ads and 1.0 mg/l NAA after six weeks of culture. The percent of regeneration varied depending on the culture medium. The elongated shoots were rooted within 9 to 11 days on ½ strength MS medium supplemented with 0.5 mg/l indole-3-butyric acid (IBA) or 1-naphthaleneacetic (NAA) acid with 2% sucrose. Maximum percentage of rooting (76.24%) was obtained on medium having 0.5 mg/l IBA and 2% sucrose. Basal region of the micro-shoots became callusing when transferred to higher concentrations of either IBA or NAA. The rooted plantlets were survived in the greenhouse. The in vitro raised plantlets were grown normally under soil condition. This result will facilitate the conservation and propagation of the important medicinal plant.

Keywords: In vitro, shoot bud regeneration, growth regulators, medicinal plants.

African Journal of Biotechnology Vol. 12(39), pp. 5738-5742