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A simple and efficient method for extraction of genomic DNA from tropical tuber crops
Abstract
DNA extraction in many plants is difficult because of metabolites that interfere with DNA isolation procedures and subsequent applications, such as DNA restriction, amplification and cloning. We have
developed a reliable and efficient method for isolating genomic DNA free from polysaccharide, polyphenols and protein contaminants from tropical tuber crops (Elephant foot yam, Cassava, Sweet
potato Taro and Tannia). The method involves inactivation of contaminant proteins by using CTAB/Proteinase K and precipitation of polysaccharides in the presence of high concentration of salt.
The purity of genomic DNA was confirmed by A260/280 and A260/230 ratios calculated from the spectrophotometric readings and further by restriction analysis of the isolated DNA using restriction enzymes EcoRI and Hind III. The described protocol also resulted in the isolation of sufficiently higher yield of DNA from leaf sample of tropical tuber crops. The new protocol can be successfully used for
both small and large scale preparation of genomic DNA from leaf tissues of tuber crops which is highly suitable for further down stream processes like PCR amplification and restriction digestion analysis.
developed a reliable and efficient method for isolating genomic DNA free from polysaccharide, polyphenols and protein contaminants from tropical tuber crops (Elephant foot yam, Cassava, Sweet
potato Taro and Tannia). The method involves inactivation of contaminant proteins by using CTAB/Proteinase K and precipitation of polysaccharides in the presence of high concentration of salt.
The purity of genomic DNA was confirmed by A260/280 and A260/230 ratios calculated from the spectrophotometric readings and further by restriction analysis of the isolated DNA using restriction enzymes EcoRI and Hind III. The described protocol also resulted in the isolation of sufficiently higher yield of DNA from leaf sample of tropical tuber crops. The new protocol can be successfully used for
both small and large scale preparation of genomic DNA from leaf tissues of tuber crops which is highly suitable for further down stream processes like PCR amplification and restriction digestion analysis.
