Volumetric and viscometric behaviour of soya bean and gram proteins in aqueous methotrexate (anticancer drug) solution at 298.15 to 308.15K

Abstract

Apparent molar volumes (V_f) and viscosities (h) for 0.00005 to 0.0004 kg.mol-1 (50 to 400 mmol kg-1) aqueous methotrexate (MT_X), gram (Gp) and soya bean (SBp) proteins, and similar compositions of Gp
and SBp each in 50 to 400 mmol kg-1 aqueous MT_X were determined at 298.15, 303.15 and 308.15K. The V_f values are positive except aqueous MT_X and listed as SBp > Gp > MTX in aqueous and SBp > Gp in MTX solutions, respectively. It infers weaker hydrophobic heteromolecular interaction of SBp in binary and ternary systems. The higher h values of SBp and Gp in MT_X than those of aqueous MT_X prove strengthening of hydrophobic interactions of proteins by MT_X. It illustrates the conformational changes of proteins; slightly higher V_f values of SBp than of Gp. The MTX confirm higher structural activity in biological process. The Vf 0 of MT_X increases with K and decrease with composition. But with compositions the values continuously decrease with lower magnitude.