Article Sidebar
Published:
Sep 10, 2010Keywords:
Camellia sinensis carotenoids phytoene synthase gene cloning sequencing phylogenetic tree.
Article Details
Issue
Section
Articles
Copyright for articles published in this journal is retained by the journal.
Main Article Content
Cloning and characterization of a cDNA encoding phytoene synthase (PSY) in tea
Abstract
Carotenoids are an important group of precursors of volatile flavour compounds in tea and phytoene synthase (PSY) is a key enzyme during the biosynthesis pathway of carotenoids. A cDNA encoding PSY
in tea shoot [Camellia sinensis (L.) O. Kuntze] was cloned and sequenced in the present study. The obtained tea PSY cDNA was 1296 bp with an open reading frame of 987 bp. The predicted protein displayed a sequence of 329 amino acids. The phylogenetic tree showed that the tea PSY was clustered closely to the PSY of Adonis palaestina, with an overall amino acid identity of 90%.
in tea shoot [Camellia sinensis (L.) O. Kuntze] was cloned and sequenced in the present study. The obtained tea PSY cDNA was 1296 bp with an open reading frame of 987 bp. The predicted protein displayed a sequence of 329 amino acids. The phylogenetic tree showed that the tea PSY was clustered closely to the PSY of Adonis palaestina, with an overall amino acid identity of 90%.
