Shoot organogenesis in oleaster (<i>Elaeagnus angustifolia</i> L.)

Abstract

Efficient plant regeneration through organogenesis was achieved from callus cultures derived from leaf explants of Elaeagnus angustifolia. Calli were obtained on MS medai containing 3% sucrose and different concentrations of TDZ (0.5, 1, 2, 3, 4 and 6 mg/l). Maximum percentage response for callus formation was 85% on MS medium supplemented with 2 mg/l of TDZ. Shoot organogenesis was achieved after transfer of calli to MS media with 3% sucrose and different concentrations of BA (0.5, 1,
2, 3, 4 and 6 mg/l) and kinetin (0.5, 1, 2, 3, 4 and 6 mg/l). Maximum organogenesis was obtained with 2 mg/l of BA. Rooting of the shoots was achieved on MS supplemented with 0.2 mg/l of IBA. Regenerated plantlets were acclimatized and successfully transplanted to soil.