Direct in vitro regeneration of a medicinal tree Oroxylum indicum (L.) Vent. through tissue culture

Abstract

A simple and reliable protocol was developed through apical and axillary bud explants of

Oroxylum indicum for multiple shoot regeneration. Effect of 2 cytokinins; BAP and KN, was studied. BAP at 4.43 μM proved better than KN with highest frequency of shoot initiation and maximum number of shoots initiated. Axillary bud showed significantly (p < 0.05) high shoot multiplication on MS medium with 4.43 μM BAP in subsequent sub culture passages. Elongated shoots were rooted on half strength MS medium with 4.92 μM IBA. Of the various methods used for hardening of in vitro regenerated plantlets, maximum plantlets survived after their subsequent transfer through sterilized-distelled-tap water and ultimately into soil:sand mixure.