Article Sidebar
DOI:
10.5897/AJB09.1046Keywords:
Phytoplasma polymerase chain reaction cloning 16SrDNA banana phylogenetic analysis
Article Details
Issue
Section
Articles
Copyright for articles published in this journal is retained by the journal.
Main Article Content
Molecular diagnostics and phylogenetic analysis of ‘Candidatus phytoplasma asteris’ (16SrI- Aster yellow group) infecting banana (<i>Musa</i> spp.)
Abstract
The presence of phytoplasma in diseased banana plants exhibiting acute stunting symptoms was detected by the polymerase chain reaction using a primer set specific for the 16SrRNA gene of phytoplasma. The amplified DNA fragments of 1500 bp were cloned in pGEMT-Easy plasmid cloning
vector and sequenced. The BLASTN and phylogenetic analyses revealed the infecting agents to be the closely related members of the ‘Candidatus phytoplasma asteris’ (16Sr I-Aster yellow) group.
vector and sequenced. The BLASTN and phylogenetic analyses revealed the infecting agents to be the closely related members of the ‘Candidatus phytoplasma asteris’ (16Sr I-Aster yellow) group.
