Main Article Content

Molecular cloning and characterization of a new peroxidase gene (OvRCI) from <i>Orychophragmus violaceus</i>


D Qilin
W Jin
F Bin
L Tingting
C Chen
L Honghui
D Shizhang

Abstract

A new peroxidase gene from Orychophragmus violaceus was cloned. The full-length cDNA of O.violaceus peroxidase gene (OvRCI, GenBank. Acc. No. AY428037) was 1220 bp and contained an 1128 bp open reading frame encoding a protein of 375 amino acids. Homology analysis and molecular
modeling revealed that OvRCI strongly resembled other peroxidase genes. Quantitative real-time PCR analysis revealed that it was a constitutively salt-inducible gene and its transcript level was most abundant after 24 h treatment with 200 mmol.L-1 sodium chloride. Our studies suggested that OvRCI was a new member of the family of recently cloned peroxidase genes.

Journal Identifiers


eISSN: 1684-5315