Isolation and identification of a cold-adapted lipase producing strain from decayed seeds of <i>Ginkgo biloba L.</i> and characterization of the lipase

Abstract

A cold-adapted lipase producing strain of mesophilic bacterium, named SYBC LIP-Y, was isolated from the decayed seeds of Ginkgo biloba L. by screening with plates containing Victoria Blue B and with the flask-shaking fermentation. It was identified as a novel Burkholderia species. The properties of its lipase after purification by PEG1000/ potassium phosphate aqueous two-phase system were characterized. The optimal pH and temperature of the enzyme was 10.0 and 30°C, respectively. About 80% of the original activity is maintained by heating at 40°C for 60 min. The lipase could also retained
70% of the maximal activity at the temperature of 0°C and suggested that it may be belonged to the cold-adapted lipase. Thus, it was proved to have good temperature stability and might have wide applicating fields.