Full Length Research Paper Seed germination and <i>in vitro</i> plant regeneration of <i>Parkia biglobosa</i> (Jacq.) Benth

Abstract

Parkia biglobosa is an important leguminous forest species which is being threatened of going into extinction in Senegal. To preserve this genetic resource of great economic value, studies on germination were carried out and in vitro conservation option through tissue culture technique was
adopted. 100% of germination rate was recorded 2 days after 5 - 6 h of concentrated H2SO4 treatments and incubation in a growth chamber at 27±1°C. To improve and raise in vitro vegetative multiplication capacities, different concentrations of growth regulators were added alone or in combination in a MS basal medium. Multiplication rate of 3.08 and 3.29 were obtained, respectively, for apex and cotyledonary nodes in a MS medium supplemented with BAP 0.5 mg.L-1 + TIBA 0.1 mg.L-1. For axillary explants, a rate of 3.58 was recorded for the hormonal combination which consisted of BAP 0.5 mg.L-1 + TIBA 0.5 mg.L-1 + AgNO3 0.5 mg.L-1. 41.66% of apex and 58.33% of cotyledonary explants were rooted after 24 h of induction treatment with IBA 2.5mg.L-1 whereas 41.66% of axillary bud explants developed roots after 48 h of induction with NAA 1mg.L-1. During acclimatization achievement, survival rates were
respectively 80% for apex and cotyledonary explants and 86.66% for axillary explants.