Confirmation of sunflower F₁ hybrids using SDS-PAGE analysis

Abstract

Among bio-chemical techniques, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDSPAGE) is most widely used due to its validity and simplicity for describing genetic structures of group of plants. In the present study, SDS-PAGE was used to confirm sunflower hybrids. Four male sterile lines, four restorer lines and their sixteen F1 hybrids were analyzed. Based on results of electrophoretic band spectra, Jaccard’s similarity index (S) was calculated for all possible pairs. The similarity matrix, thus, generated was converted to a dissimilarity matrix and used to construct dendrogram, using unweighed pair-group method with arithmetic means. Euclidean distance of 1.0 was observed among 5 comparisons, whereas maximum distance (3.87) was observed for 1 comparison only. In most of the cases, female parents, along with their respective crosses, were found in one cluster, indicating that
SDS-PAGE can be used reliably for the identification of hybrids in sunflower.