The effect of carbon dioxide at high pressure under different developmental stages of <i>Callosobruchus maculatus</i> (F.) hosting on chickpeas

Abstract

In this paper, the minimum exposure time required to kill Callosbruchus maculatus (F.) in all the developmental stages of 99.90% purity carbon dioxide (CO₂) under 20 bars at 23 to 26°C was determined. Adults, pupae, larvae and eggs of C. maculatus hosting kabuli type organic chickpeas were exposed to CO₂ for 240 min. The minimum exposure time for killing C. maculatus was found to be 180 min in all its developmental stages. As a consequence, one minute exposure time was sufficient to kill the adults of C. maculates. However, it was determined that the pupae stage was more tolerant to high pressure CO₂.

Key words: Callosabruchus maculatus, high pressure, carbon dioxide, organic chickpea.