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A comparative evaluation of rehydration and cuploading sample application for modified twodimensional gel electrophoresis of human serum proteins using immobilized pH gradient


A Mostafaie
K Yari
S Kiani

Abstract

Proteomics is a powerful technique to study proteomes extracted from biological sources. Proteome analysis classically is based on two-dimensional electrophoresis (2D-E) for protein separation and mass spectrometry (MS) for the protein identification. The serum protein analysis is a useful diagnosis that can be used as an indicator of the physiological or clinical status of a patient. One of the central and critical steps is sample application, therefore this method should be considered and optimized for 2-DE with immobilized pH gradient. The aim of this study was focused on comparative application of ingel (rehydration loading) and in-cup (cup loading) sample application for 2-DE of human serum analysis. The results indicate that the number of spots detected with the rehydration loading was higher than the number obtained by cup-loading sample application. Also in this work, in order to obtain the best result with high resolution, a 2-DE procedure was optimized.

Key words: Proteomics, 2D-E, proteome, sample application.


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eISSN: 1684-5315