Efficient plant regeneration through somatic embryogenesis in sugarcane (Saccharum officinarum L.) cultivar CPF-237
In the present experiment, a number of cultures were established for optimization of normal plant regeneration in sugarcane (Saccharum officinarum L.) cv., CPF-237. Well callus induction as well as its proliferation was observed on Murashige and Skoog (MS) medium supplemented with 3.0 mg L-1 2,4- dichlorophenoxyacetic acid (2,4-D) and 2.0% sucrose. Almost seven-weeks old proliferated calluses were sub-cultured on MS3 [MS, 0.4 mg L-1 kinetin (kin), 0.5 mg L-1 benzyleaminopurine (BAP), 0.3 g L-1 casein hydrolysate, 3% sucrose] medium for somatic embryogenesis under dark condition for fourweeks. Greenish plantlets were regenerated (8.0 plantlets per callus) on MS5a (MS, 0.2 mg L-1 kin, 0.3 mg L-1 BAP, 3.0% glucose) medium in six-weeks under light conditions. Regenerated plantlets were not variant morphologically and rooted on MS7 (MS, 0.1 mg L-1 indole-3-butyric acid (IBA) medium in 1½ weeks.
Key words: Saccharum officinarum L., shoot tips culture, callusing, somatic embryogenesis, glucose, normal plantlets, rooting.