Characterization of pseudorabies viruses produced in mammalian cells by viral genome transfection

Abstract

The full-length genome DNA of pseudorabies virus (PrV) was transfected into swine testis (ST) cells using calcium phosphate method to generate the infectious viruses. The resulting viruses were identified by indirect immunofluorescence and were used to infect African green monkey kidney (Vero) cells. Our results showed that the produced viruses had similar plaque morphology and growth properties with PrV vaccine strain, Bartha-K61. This method may produce PrV at an inexpensive cost.

Key words: Pseudorabies virus, transfection, virus plaque, cytopathic effect.