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African Journal of Biotechnology

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Examination of genetic diversity in common bean (Phaseolus vulgaris L.) using random amplified polymorphic DNA (RAPD) markers

A Bukhari, MA Bhat, M Ahmad, N Saleem

Abstract


To study the pattern of genetic diversity in 45 genotypes of common bean, 19 RAPD primers were used. Of 253 bands produced, 236 bands (94.22%) were polymorphic in which maximum number (20 polymorphic bands) were observed in the profiles of the primer OPB-07. Highest PIC value (0.79) was observed for the primers OPG-14 and OPE-1, whereas lowest PIC value (0.34) was recorded for the primer OPA-11 with an average PIC value of 0.54. Similarly, highest effective multiplex ratio value (0.11) was scored for the primers OPA-04 and OPG-6 and the lowest value (0.05) was recorded for the primer OPB-07. Primer OPG-14 and OPE-1 exhibited the highest marker index value (0.078) and the lowest value (0.024) was recorded for OPA-11. Pair-wise genetic similarity coefficients of genotypes varied from 0.56 to 0.92 with an average of 0.70 amongst 45 genotypes. The maximum similarity coefficient (0.92) was noticed between G-9 and G-8. Cluster analysis separated 45 genotypes into seven major clusters which were further grouped into various sub-clusters. Among these seven clusters, the maximum number of genotypes (12) were recorded in cluster I, while cluster VII formed a mono-genotypic cluster. 

Key words: Genetic diversity, polymorphism, cluster analysis, random amplified polymorphic DNA markers (RAPD), common bean.




http://dx.doi.org/10.5897/AJB2014.14281
AJOL African Journals Online