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African Journal of Biotechnology

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Detection of Pseudomonas fluorescens from broth, water and infected tissues by loop-mediated isothermal amplification (LAMP) method

P Saharan, JS Duhan, SK Gahlawat

Abstract


Loop mediated isothermal amplification is rapid, highly sensitive and specifically developed method for detection of bacterial infections. AprX gene for alkaline metalloprotease of Pseudomonas fluorescens was used to design four primers and loop mediated isothermal amplification (LAMP) conditions were standardized for amplification of DNA. LAMP primers successfully amplified P. fluorescens from DNA and bacterial cells taken directly from broth, water and infected tissues with high specificity and sensitivity (10 pg) under isothermal condition at 61°C.


Key words: Pseudomonas fluorescens, loop mediated isothermal amplification (LAMP), rapid, simple, specificity, sensitivity.




http://dx.doi.org/10.5897/AJB2014.14360
AJOL African Journals Online