Laboratory evaluation of freshly prepared juice from garlic (Allium sativum L.) Liliaceae as protectants against the maize weevil, Sitophilus zeamais (Motsch.) [Coleoptera: Curculionidae]

  • Ifeanyi D Nwachukwu
  • Elechi F Asawalam

Abstract

Fresh prepared garlic (Allium sativum L.) juice, containing the antimicrobial allicin, was evaluated as a possible grain protectant against the maize weevil, Sitophilus zeamais (Motsch.). Each experiment was set out in completely randomized design (CRD) with four replications and a control treatment. Adult mortality and percentage weight loss were investigated. There was an observed increase in adult mortality following days of exposure in all treatments. Statistically significant (P<0.05) reduced grain loss was also observed in all the treatments when compared with the control. The juice prepared from an indigenous Nigerian garlic cultivar (GUN) was more lethal (causing 93% adult mortality), when applied topically on the freshly emerged S. zeamais adults, compared to the juice prepared from a clove of garlic purchased at a supermarket in Germany (GAG). High performance liquid chromatography (HPLC) analysis indicated that the amount of allicin in GUN was 1883.2 μg/ml while that in GAG was 3500.93 μg/ml. This study highlights the potential of A. sativum containing allicin for biorational control of maize grains against S. zeamais infestation and damage.

Keywords: Allicin, Allium sativum, biopesticide, biorational control, crop protectant, Sitophilus zeamais, stored product

African Journal of Biotechnology, Vol. 13(10), pp. 1123-1130, 5 March, 2014

Author Biographies

Ifeanyi D Nwachukwu
Department of Plant Physiology, Institute for Biology, RWTH Aachen University, Worringerweg 1, D-52056 Aachen, Germany; Department of Human Nutritional Sciences, University of Manitoba, W542-190 Dysart Road, Winnipeg, Canada MB R3T 2N2
Elechi F Asawalam
Department of Plant Health Management, Michael Okpara University of Agriculture, Umudike P.M.B 7267 Umuahia, Abia state Nigeria
Published
2015-05-27
Section
Articles

Journal Identifiers


eISSN: 1684-5315