Identification and characterization of milk-clotting proteases produced by two species of mold

  • Aicha Benlounissi
  • Aicha Mechakra-Maza
  • Loic J Blum
  • Christophe A Marquette

Abstract

Two strains of fungi were isolated and identified as Aspergillus tamarii and Penicillium pinophilum which showed good enzymatic activity on casein, 1933.33U and 1822, 21U, respectively. The search for milk clotting enzymes by fermentation at acid pH on culture medium containing whey and, after purification by molecular exclusion chromatography, affinity chromatography and SDS-PAGE helped to find two enzymes having a coagulant activity and approximately molecular weight of 35 and 30 KDa for Aspergillus tamarii and Penicillium pinophilum, respectively. The optimum pH of activity was 5.5 for both strains and the optimum temperature was 50 and 35°C for A. tamarii and P. pinophilum, respectively.

Keywords: Aspergillus, Penicillium, milk-clotting enzyme, extraction and purification

African Journal of Biotechnology, Vol 13(11), 1275-1280

Author Biographies

Aicha Benlounissi
Department de Biologie, Faculte des Sciences, Universite 20 Aout 1955, Skikda, Algerie - Laboratoire de Biologie de l'environnement, Department de Biochimie-Microbiologie. Faculte des Sciences de la nature et de la vie, Universite Mentouri Constantine, Algerie
Aicha Mechakra-Maza
Laboratoire de Biologie de l'environnement, Department de Biochimie-Microbiologie. Faculte des Sciences de la nature et de la vie, Universite Mentouri Constantine, Algerie
Loic J Blum
Equipe Genie Enzymatique, Membranes Biomimetiques et Assemblages Supramoleculaires, Institut de Chimie et Biochimie Moleculaires et Supramoleculaires, Universite Lyon 1 - CNRS 5246 ICBMS, Batiment CPE -43, bd du 11 novembre 1918 - 69622 Villeurbanne, Cedex, France
Christophe A Marquette
Equipe Genie Enzymatique, Membranes Biomimetiques et Assemblages Supramoleculaires, Institut de Chimie et Biochimie Moleculaires et Supramoleculaires, Universite Lyon 1 - CNRS 5246 ICBMS, Batiment CPE -43, bd du 11 novembre 1918 - 69622 Villeurbanne, Cedex, France
Published
2015-06-10
Section
Articles

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eISSN: 1684-5315