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Gibberellic acid, amino acids (glycine and L-leucine), vitamin B<sub>2</sub> and zinc as factors affecting the production pigments by <i>Monascus purpureus</i> in a liquid culture using response surface methodology

Fateme Baneshi
Majid Azizi
Mohsen Saberi
Mohammad Farsi


The combined effects of zinc, gibberellic acid, vitamin B2, amino acids (glycine and L-leucine) on pigment production were evaluated in a liquid culture of Monascus purpureus. In this study, response surface design was used to optimize each parameter. The data were analyzed using Minitab 14 software. Five parameters were applied by using response surface methodology for pigment production in liquid cultures. Four zinc, 11 gibberellic acids, three vitamins B2, eleven and fifteen amino acids (glycine and L-leucine) respectively; levels were evaluated. The highest production of pigment was reached with a 10 mg/l vitamin B2, 50 mg/l gibberellic acid and 50 mg/l glycine amino acid.

Keywords: Monascus purpureus, response surface methodology, amino acid, gibberellic acid, zinc, vitamin B2

African Journal of Biotechnology, Vol 13(12), 1484-1490

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eISSN: 1684-5315