Fruit, seed and embryo development of different cassava (Manihot esculenta Crantz) genotypes and embryo rescue

  • Huabing Yan
  • Liuying Lu
  • Adriana Alzate
  • Hernan Ceballos
  • Clair Hershey
  • Songbi Chen
  • Kainmian Li

Abstract

Fruit, seed and embryo developments of different cassava (Manihot esculenta Crantz) genotypes, as well as embryo rescue, were investigated. The fruits of three genotypes after uncontrolled open pollination presented the same progressive development with similar sizes at different stages. There are large differences in the fruit set as well as the embryo development between different genotypes. Days after pollination (DAP) was found not to be an adequate predictor of embryo size as their size ranged from almost invisible to 8.7 mm in length at 32 DAP even within the different locules of the same fruit. The ideal stage for embryo rescue in cassava was from 32 to 36 DAP, because at that stage most embryos are visible (> 0.7 mm); and their excision without injury is feasible. Also, in vitro germination of the cotyledonary embryos at that stage had a high success rate. A half Murashige and Skoog (MS) medium supplemented with 1.0 mg/l GA3, 2% sucrose and 0.2% gel rite proved to be adequate for embryo rescue.

Keywords: Manihot esculenta Crantz, day after pollination (DAP), fruit set, seed size, embryo size, embryo rescue

African Journal of Biotechnology, Vol 13(14), 1524-1528

Author Biographies

Huabing Yan
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), Danzhou, PR, China; Cash Crop Research Institute, Guangxi Academy of Agricultural Sciences (GXAAS), Nanning, PR, China
Liuying Lu
Cash Crop Research Institute, Guangxi Academy of Agricultural Sciences (GXAAS), Nanning, PR, China
Adriana Alzate
International Center for Tropical Agriculture (CIAT), km. 17 Recta, Cali-Palmira, Colombia
Hernan Ceballos
International Center for Tropical Agriculture (CIAT), km. 17 Recta, Cali-Palmira, Colombia
Clair Hershey
International Center for Tropical Agriculture (CIAT), km. 17 Recta, Cali-Palmira, Colombia
Songbi Chen
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), Danzhou, PR, China
Kainmian Li
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), Danzhou, PR, China
Published
2015-06-25
Section
Articles

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eISSN: 1684-5315