Main Article Content

Use of plumules cryopreservation to save coconut germplasm in areas infected by lethal yellowing


Oula Alla-N'Nan
Sery Gonedele Bi
Kouakou Tiecoura
Jean-Louis Konan Konan

Abstract

Plumules excised from zygotic embryos through the largest representative diversity of four of the five different areas of coconut cash and food crops were used in a cryopreservation process using encapsulation-dehydration technique. Five accessions of coconut trees were used [Panama Tall (PNT/GPA), Brazilian Green Tall (BGD/NVB), Cameroon Red Dwarf (CRD/NRC), Vanuatu Tall (VTT/VNT/GVT), and Tagnanan Tall (TAGT/GTN)] in addition to the accession model [Malayan Yellow Dwarf (MYD)] from which an optimal protocol was obtained. A great variability of response was observed depending on accessions with survival and growth recovery rates varying from 6 to 66% and 0 to 24% after 2 and 7 months of culture, respectively.

Keywords: Coconut, accessions, germplasm, plumules, cryopreservation, encapsulation-dehydration

African Journal of Biotechnology, Vol 13(16), 1702-1706

Journal Identifiers


eISSN: 1684-5315