Certain growth related attributes of bunchy top virus infected banana under ex-vitro conditions

  • Ikram ul-Hag
  • Shahrukh Shahrukh
  • Um E-Aiman
  • Saifullah Khan
  • Nazia Parveen
  • Madiha Fatima
  • M U Dahot

Abstract

Effect of banana bunchy top virus (BBTV) on morpho-physiological characteristics of banana (Musa sp.) cv., Basrai plants was assessed. Healthy and BBTV infected samples of banana were collected from its open fields and micro-propagated aseptically. These plantlets were established in wire-house for three months. Presence of BBTV was confirmed by polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Micro-propagation efficiency of BBTV-infected plants was observed in less than healthy plants under in-vitro as well as in-vivo. Significant reduction in plant height, fresh weight (F wt) and dry weight (D wt) of leaf was observed in BBTV infected plants. A co-relation of peroxidase (POX) activity was observed with total carotenoids that increased in BBTV infected plants, while chlorophyll contents decreased significantly. Nitrate reductase activity also decreased with increase in proline contents in BBTV-stresses plants (p<0.05). Meanwhile, reducing sugars also increased but not-significantly. Bunchy top virus infection in banana therefore resulted in alteration of growth related physiological traits that led to retardation of plant growth.

Keywords: Ex-vitro, Musa sp., micro-propagation, photosynthetic pigments, BBTV, peroxidases, total proteins, reducing sugars, cell size

African Journal of Biotechnology, Vol 13(18), 1876-1882

Author Biographies

Ikram ul-Hag
Institute of Biotechnology  and Genetic Engineering (IBGE) University of Sindh, Jamshoro-76080, Pakistan
Shahrukh Shahrukh
Institute of Biotechnology  and Genetic Engineering (IBGE) University of Sindh, Jamshoro-76080, Pakistan
Um E-Aiman
Institute of Biotechnology  and Genetic Engineering (IBGE) University of Sindh, Jamshoro-76080, Pakistan
Saifullah Khan
Plant Tissue Culture and Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi-75270, Pakistan
Nazia Parveen
Department of Statistics, University of Sindh, Jamshoro-76080, Pakistan
Madiha Fatima
Institute of Biotechnology  and Genetic Engineering (IBGE) University of Sindh, Jamshoro-76080, Pakistan
M U Dahot
Institute of Biotechnology  and Genetic Engineering (IBGE) University of Sindh, Jamshoro-76080, Pakistan
Published
2015-07-10
Section
Articles

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eISSN: 1684-5315