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African Journal of Biotechnology

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Molecular markers linked to apomixis in Panicum maximum Jacq.

AC Bluma-Marques, L Chiari, DC Agnes, L Jank, MS Pagliarini

Abstract


Panicum maximum Jacq. is an important forage grass of African origin largely used in the tropics. The genetic breeding of this species is based on the hybridization of sexual and apomictic genotypes and selection of apomictic F1 hybrids. The objective of this work was to identify molecular markers linked to apomixis in P. maximum to determine easily and at an early stage, the reproductive mode of F1 hybrids, so to assist the breeding program. A bulked segregant analysis was performed using 184 random amplified polymorphic DNA (RAPD) primers in an F1 population of P. maximum segregating for reproductive mode. Four RAPD markers linked to apomixis were identified and mapped in this population. These markers showed good selection efficiency, ranging from 77.3 to 88%, with 81.3% when analyzed together. Two of these markers were easily transferred to another F1 population of P. maximum. In this population, the selection efficiency was also high for both markers; 84.8 and 90%, when analyzed together. These markers may be used in the assisted selection for reproductive mode in both F1 progenies of P. maximum studied here and in other populations to which they can be transferred.

Keywords: Guinea grass, apospory, reproduction mode, random amplified polymorphic DNA (RAPD), selection efficiency.

African Journal of Biotechnology, Vol 13(22) 2198-2202



http://dx.doi.org/10.5897/AJB2014.13703
AJOL African Journals Online